TABLE 1.
ATPase activity
ATPase activity of 0.1 μm katanin p60 with 1 mm ATP at 25 °C was measured in the absence or presence of 1 μm MTs by a malachite green assay (18). The mean ATPase activities ± S.E. were determined from at least three independent experiments. ATPase activities of ∼5 nmol/nmol/min were considered background levels (*) under the experimental conditions used since ATPase-negative mutants were also measured to have these levels of activity.
| Katanin p60 | MT | ATPase activity |
|---|---|---|
| nmol/nmol/min | ||
| Wild type | − | *4.36 ± 0.57 |
| Wild type | + | 39.7 ± 3.95 |
| K280A | + | *5.04 ± 0.67 |
| E334Q | + | *5.61 ± 3.87 |
| K306A | − | *5.65 ± 0.29 |
| K306A | + | 80.6 ± 3.63 |
| Y307A | − | *5.68 ± 0.63 |
| Y307A | + | 11.7 ± 3.42 |
| H308A | − | *5.46 ± 1.22 |
| H308A | + | 66.1 ± 14.36 |