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. 2015 May 1;11(5):e1005210. doi: 10.1371/journal.pgen.1005210

Fig 3. MBD7 prevents the silencing of a report gene and some endogenous genes.

Fig 3

(A) The mbd7-1 mutation causes the silencing of 35S-NPTII but not the RD29A-LUC transgene. Seedlings grown in MS plates were imaged after cold treatment at 4°C for 24 h. For kanamycin resistance test, the seeds were planted on MS medium supplemented with 50 mg/L kanamycin and incubated for 2 weeks before being photographed. The reporter genes were introduced to mbd7-1 mutant plants by crossing. (B) Real-time PCR analysis of the expression level of LUC and NPTII reporter genes in the different genotypes. (C-E) Effect of mbd7 mutation on the expression of endogenous genes. Left column: Snapshot in the IGB showing DNA methylation levels in ros1-4, idm1-1, mbd7-1 mutant plants and wild type control; right column: real-time PCR analysis of the expression level of the hypermethylated genes or nearby genes in different genotypes. TUB8 was used as an internal control. Error bars represent standard error (n = 3).