Fig 2. cSrc is the most upstream molecule involved in P4-induced increases in the levels of p21^cip1 and p27^kip1.

Pre-treatment with PP2 (200 nM) for 1 h prevented P4-induced membrane translocation of Kras (A), increases in the levels of p-Raf-1, p-ERK1/2, p-AKT and p-p38 protein (B), and p21^cip1, p27^kip1 and p53 protein (C). Data are representative of 2 independent experiments with similar results. Values shown in parentheses represent the quantified results adjusted with G3PDH and cadherin for cytosol and membrane, respectively (A), with their own total protein level (B), or with α-tubulin (C) and expressed as ratio over control. Con, control.