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. 2015 May 1;10(5):e0125903. doi: 10.1371/journal.pone.0125903

Fig 5. The cSrc/Kras/Raf-1/AKT/ERK/p38-mediated pathway is involved in P4-induced NFκB (p65) nuclear translocation.

Fig 5

(A) Pre-treatment of RASMCs with PP2 (200 nM) for 1 h prevented the P4-induced NFκB nuclear translocation. Pre-treatment of RASMCs with 200 nM PP2 (B), 1 μM SB 203580 (C), or 100 nM wortmannin (D) prevented P4-induced increases in the levels of p-IκBα. (E) Pre-transfection with dn-AKT cDNA prevented P4-induced increases the levels of p53, p21cip1 and p27kip1 protein. (F) Pre-treatment with BAY (10 nM) prevented P4-induced increases in the levels of p53, p21cip1 and p27kip1 protein. (A-F) Data are representative of 2 independent experiments with similar results. Values shown in parentheses represent the quantified results adjusted with their own total protein level, RARP (for nuclear protein) or G3PDH (for cytosolic protein) and expressed as ratio over control. (G) Pre-transfection with dn-p53 cDNA prevented P4-induced increases in the levels of p21cip1and p27kip1 protein. Values shown in parentheses represent the quantified results adjusted with α-tubulin protein level and expressed as ratio over control. Values represent the means±s.e.mean. (n = 3). *P < 0.05 different from control group. BAY, BAY11-7082; Con, control; C3.1 (+), pcDNA 3.1 (+); dn, dominate negative; SB, SB 203580; WM, wortmannin.