Table 2. Stress-dependent alteration of metabolite concentrations after 20 min as determined by GC/MS and comparison with H2O2 exposure.
| Metabolite | Tendency after HOCl stress | Return to initial concentration | Tendency after H2O2 determined by Jozefczuk et al. [23] |
|---|---|---|---|
| a) Metabolites that show similar behavior upon HOCl and H 2 O 2 stress | |||
| Aspartate | ↑ | yes | ↑ |
| Glutamate | ↑ | yes | ↑ |
| Phenylalanine | ↑ | yes | ↑ |
| Threonine | ↑ | yes | ↑ |
| Pyroglutamic acid | ↑ | no | ↑ |
| b) Metabolites that do not alter upon both stress conditions | |||
| Proline | Ø | no | Ø |
| Putrescine (1,4-Diaminobutane) | Ø | no | Ø |
| Pelargonic acid | Ø | no | Ø |
| Palmitic acid (Decanoic acid) | Ø | no | Ø |
| Palmitelaidic acid (9-trans Decenoic acid) | Ø | no | Ø |
| c) Metabolites that show different alterations or were not identified by Jozefcuk et al. | |||
| Succinate (Butanedioic acid) | ↑ | yes | Ø |
| Urea | ↑ | yes | Ø |
| Phosphate | ↑ | no | Ø |
| Decanamide (Capramide) | ↑ | no | n.d |
| 1-Monopalmitoylglycerol | ↑ | no | n.d |
| 2-Monopalmitoylglycerol | ↑ | no | n.d |
| 1-Monooleoylglycerol | ↑ | no | n.d |
| Glycine | Ø | no | ↑ |
| Leucine | Ø | no | ↓ |
| Valine | Ø | no | ↓ |
| Tyrosine | Ø | no | ↑ |
| Tetradecanoic acid (Myristic acid) | Ø | no | n.d |
| Pentadecanoic acid | Ø | no | n.d |
| Alanine | ↓ | no | ↑ |
| Methionine | ↓ | no | Ø |
| Lactic acid | ↓ | no | n.d |
| 2-Aminobutyric acid | ↓ | no | ↑ |
| Pyrimidine | ↓ | yes | n.d |
| Phosphoglycerol | ↓ | yes | Ø |
| 2-Hydroxybutyric acid | ↓ | no | Ø |
| Orotic acid | ↓ | no | Ø |
| Glycolic acid | ↓ | no | Ø |
| Stearic acid (Octadecanoic acid) | ↓ | yes | Ø |
Overview of the concentration alterations after HOCl exposure of metabolites included in the discussion of the main text. Comparison of metabolite levels of a previous study by Jozefczuk et al. who analyzed the metabolite alteration after H2O2 exposure. Alterations of the concentrations are indicated by arrows (↑ increase; ↓ decrease). For detailed information, see S2 Table.