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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Mol Cell Biochem. 2015 Mar 6;404(0):171–179. doi: 10.1007/s11010-015-2376-4

Figure 4.

Figure 4

Changes of COMP fragments, MMP13 and inflammatory cytokines level in arthritic TG mice. (A) Sandwich ELISA was performed to examine the concentration of COMP fragments in serum. (B) Real-time PCR analysis of MMP13 mRNA expression. (C) Representative IHC sections of hindpaws joints. Scale bars, 50μm. (D) Real-time PCR analysis of TNF-α mRNA expression. (E) Real-time PCR analysis of IL-17 mRNA expression. (F and G) ELISA was performed to examine the levels of IL-17 and TNF-α in serum. (H) Intracellular FACS staining of TNF-α and IL-17 in draining lymph node cells. All data represent three independent results. *P < 0.05, **P<0.01.