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. Author manuscript; available in PMC: 2016 Apr 28.
Published in final edited form as: Cell Rep. 2015 Apr 16;11(4):551–563. doi: 10.1016/j.celrep.2015.03.045

Figure 6. Visualization of KASH5-SUN1 Complexes Coupling Telomeres to dynein on Cytoskeletal Microtubules.

Figure 6

A. Example of wild-type pachytene spermatocytes showing co-localization of components of the system that supports RPMs. The magnified areas highlight the association of the chromosome ends and associated protein complexes with microtubules. Arrows indicate dynein-SUN1 co-localization. Magnification bar represents 5μm.B. Quantitation of the co-localization of protein immunosignals in wild-type spermatocytes. C. Example of pachytene chromosome proximal telomeric ends connected to SUN1/KASH5 nuclear envelope bridges. SYCP3 was used to visualize chromosome cores and CREST marks the proximal telomeric ends. D. Proposed model for meiotic chromosome telomere-nuclear envelope attachment and connection to dynein on microtubules. E. Schematic representation of zygotene nuclei. The proposed model in D and E summarizes observations of this and previous studies. In E arrows represent the direction of telomere movements. At 1, RPMs disrupt a non-homologous unproductive interaction. At 2, RPMs facilitate interlock resolution. See also Figure S4.