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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Neurobiol Aging. 2015 Feb 18;36(5):1924–1937. doi: 10.1016/j.neurobiolaging.2015.02.011

Figure 7. Heat shock proteins and co-chaperones in neo- and allocortex as a function of age in vivo.

Figure 7

Female rats were sacrificed at 2–3.9, 4–6, 8–9, 16–18.9, and 19–22 months of age. Heat shock proteins Hsp90, Hsc70, Hsp70, HO1, Hsp25, Hsp60, and Hsp40 (A, E, F, G, H, I, J) and co-chaperones CHIP, Hip, and Hop (B, C, D) were measured in sensorimotor neocortex and entorhinal allocortex by infrared immunoblotting. Allocortical tissue exhibited higher levels of HO1 at multiple ages and higher levels of Hip at the youngest age. Age-related changes (usually increases) in Hsp90, CHIP, Hip, Hsp25 and Hsp70 were also apparent. Allocortical tissue exhibited lower levels of Hsp90 and CHIP than neocortical tissue in the oldest group, but allocortex and neocortex expressed equivalent levels of Hsp70 at all ages. n= 3–6 animals per group. * p ≤ 0.05, *** p ≤ 0.001 vs neocortex, + p ≤ 0.05, ++ p ≤ 0.01, +++ p ≤ 0.001 vs 2–4 mo., # p ≤ 0.05 vs 4–6 mo., ^ p ≤ 0.05, ^^ p ≤ 0.01 vs 8–9 mo., ~ p ≤ 0.05, ~~~ p ≤ 0.001 vs 19–22 mo., LSD post hoc correction following two-way ANOVA.