Table 4.
DiCre-recombinase inducible knockouts in T. gondii effects on egress and invasion phenotypes.
| Induced Knockout |
Egress | Invasion | Gliding | Compensatory Mechanism | Reference |
|---|---|---|---|---|---|
| MyoA | 2% | 16% | 37% | MyoC glideosome | Egarter et al., 2014 |
| MyoB/C | 100% | 100% | 100% | MyoA glideosome | Egarter et al., 2014 |
| MyoA/B/C | 2% | 5% | N.D. | MyoD? MyoE? (untested) | Egarter et al., 2014 |
| MLC1 | 5% | 28% | 42% | MyoD-MLC2? (untested) | Egarter et al., 2014 |
| GAP45 | 4% | 6% | 100% | GAP80 over-expression Frenal et al., 2014b | Egarter et al., 2014 |
| GAP80* | 85% | 80% | N.D. | GAP45 | Frenal et al., 2014b |
| GAP40 | N.D. | N.D. | N.D. | essential | Egarter et al., 2014 |
| GAP50 | N.D. | N.D. | N.D. | essential | Egarter et al., 2014 |
| Actin 1 | 2% | 10% | 10% | ? | Egarter et al., 2014 |
| Aldolase** | N.D. | 100% | 100% | GAPDH? | Shen and Sibley, 2014 |
| AMA1 | N.D. | ~25% | 100% | Other adhesins | Shen and Sibley, 2014 |
*
Phenotype from GAP80 clonal KO line with GAP45 iKO in absence of ATc. All other clones are inducible knockouts via the DiCre-recombinase system established in T. gondii.
**
Performed in low glucose conditions to alleviate toxicity of glycolysis intermediates.