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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Mol Cell Biochem. 2015 Mar 13;404(0):53–77. doi: 10.1007/s11010-015-2366-6

Fig. 2.

Fig. 2

Isolation of the L. donovani (1SCL-2D) invertase using FPLC fractionation. Concentrated supernatants from L. donovani (1SCL-2D) promastigote release assays were subjected to FPLC on a Mono-Q column. Individual fractions from this column were collected, optically scanned and their absorbance at 220 and 280 nm were plotted (a). One of these fractions (INV) when subjected to SDS-PAGE and silver staining (b) contained only a single ~70 kDa protein band (arrow). (Color figure online)