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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Mol Cell Biochem. 2015 Mar 13;404(0):53–77. doi: 10.1007/s11010-015-2366-6

Fig. 5.

Fig. 5

Detection of mRNAs encoding invertase in both Leishmania sp. promastigote and axenic amastigote developmental forms. a Northern blot of total RNA isolated from L. donovani (Ld) promastigotes (Pro) and axenic amastigotes (AxAm) probed with the LdINV-DIG858 probe. Molecular mass markers in kb are shown at the left (arrows). b Ethidium bromide stained gel showing equivalent amounts of Pro and AxAm RNA were used to generate the Northern blot shown in a above. Molecular mass markers in kb are shown at the left (arrows). c Ethidium bromide-stained agarose gel showing the ~500 bp product (arrow) obtained from RT-PCR reactions using RNA isolated from L. mexicana (Lmex) promastigotes (Pro) and axenic amastigotes (AxAm). This image was inverted using Adobe Photoshop CS2 to accentuate the RT-PCR product obtained in these reactions