Table 1.
Composition of normal and experimental diets
| Diet (ingredients) | % | Normal diet (g/kg dry matter) | Vitamin B6-deficient diet (g/kg dry matter) |
|---|---|---|---|
| Casein | 14.00 | 140 | 140 |
| Dextrinized cornstarch | 15.50 | 155 | 155 |
| Sucrose | 10.00 | 100 | 100 |
| Corn starch | 46.60 | 466 | 466 |
| Alphacel, non-nutritive bulk | 5.00 | 50 | 50 |
| Soybean oil | 4.00 | 40 | 40 |
| AIN-93M mineral mixa | 3.50 | 35 | 35 |
| l-Cystine | 0.18 | 1.80 | 1.80 |
| AIN-93-VX vitamin mixb | 1.00 | 10 | – |
| AIN-93-VX vitamin mix (without pyridoxine-HCl) | 1.00 | – | 10 |
| Choline bitartrate | 0.25 | 2.50 | 2.50 |
| tert-Butylhydroquinone | 0.08×10−2 | 0.08×10−1 | 0.08×10−1 |
All compounds used in the study were of analytical grade.
Supplied (g/kg diet), CaCO3, 357.00; KH2PO4, 250.00; K3C6H5O7·H2O, 28.00; NaCl, 74.00; K2SO4, 46.60; MgO, 24.00; Ferric citrate, 6.06; ZnCO3, 1.65; MnCO3, 0.63; CuCO3, 0.30; KIO3, 0.01; Na2O3Se, 10.25×10−3; (NH4)6Mo7O24·4H2O, 7.95×10−3; Na2O3Si·9H2O, 1.45; KCr(SO4)2·12H2O, 2.75×10−1; LiCl, 1.74×10−2; B(OH)3, 8.15×10−2; NaF, 6.35×10−2; NiCO3, 3.18×10−2; NH4VO3, 0.66×10−2; powdered sugar, 209.81.
Supplied (g/kg diet), nicotinic acid, 3; D-calcium pantothenate, 1.6; pyridoxine-HCl, 0.7; thiamine HCl, 0.60; riboflavin, 0.60; folic acid, 0.20; D-biotin, 0.02; vitamin B-12 (0.1% triturated in mannitol), 2.50; α-tocopherol powder (250 U/g), 30.00; vitamin A palmitate (250,000 U/g), 1.60; vitamin D-3 (400,000 U/g), 0.25; phylloquinone, 0.75×10−1; powdered sucrose, 959.66.