Table 2.
Involvement of Differentially Expressed Proteins in Neural Networks and Functions Identified by Ingenuity Pathway Analysis
| Networks | Focus Proteins | Protein Network | |
|---|---|---|---|
| Cell Signaling and Interaction, Nervous System Development and Function, Behavior | 14 | AK1↓, ALDH1L1↑, ALDOA↓, APP, ATP, BASP1↑, CAMK2A↑, CAP1↓, CAV1, CDK5R1, DSCAM↓, EEF2↑, EGFR, EIF2AK2, ENO1↓, F2R, FABP3, FMR1, GDI1, GRIA1, GRIA2, HK1↑, HSP90AA1↑, HSPA8↓, IFNG, MAP1B, MAP2K1, MAP2K2, MECP2, MMP9, NRGN, NSF↑, PGAM1↓, progesterone, SNAP25 | |
| Cell Morphology, Cell Assembly and Organization, Cell Development | 8 | ADAM22↑, APP, CAMK2A↑, CDK5R1, DAB1, DCX, DLG2, DLG4, DPYSL2↓, GNB2↓, GRIN2A, GRIN2B, HTR2C, INA, KCNA2, KIF5B, KIF5C↑, KLC1, KLC2, LGI1, MAP1B, MBP, MT3, NEFL, NF1, NOS1, NSF↑, PAFAH1B1↓, PAFAH1B2, RAC1, RHOA, RTN4, RTN4R, STXBP1↑, TNIK | |
| Diseases and Disorders | Function | p Value | Proteins |
| Developmental Disorder | Delay of brain development | .002 | PAFAH1B1↓ |
| Neuropsychiatric Disorder | Bipolar disorder | .009 | CAMK2A↑, DPYSL2↓ |
| Schizophrenia | .02 | DPYSL2↓, MDH1↓, PGAM1↓ | |
| Depression | .03 | CAMK2A↑, DPYSL2↓, ENO1↓, HK1↓ | |
| Molecular and Cellular | Function | p Value | Proteins |
| Cellular Development, Function, and Maintenance | Microtubule dynamics Formation of cellular projections | .004 .001 |
AK1↓, ADAM22↑, BASP1↑, CAMK2A↑, DPYSL2↓, DSCAM↓, HSPA8↓, HSP90AA1↑, PAFAH1B1↓, KIF5C↑ |
| Cell-To-Cell Signaling | Synaptic transmission and plasticity, LTP | .004 | CAMK2A↑, DPYSL2↓, EEF2↑, KIF5C↑, NSF↑, PAFAH1B1↓, STXBP1↑ |
| Metabolism | Alcohol catabolic process Glucose catabolic process |
.00001 3.0E-6 |
ALDOA↓, ENO1↓, HK1↑, MDH1↑, PGAM1↓ |
| Top Canonical Pathways | p Value | Proteins | |
| Glucogenesis/Glycolysis | 2.0E-6 | ALDOA↓, ENO1↓, HK1↑, MDH1↓, PGAM1↓ | |
| Krebs Cycle | .002 | FH↓, MDH↓ | |
| Top Upstream Regulators | p Value | Target Proteins in Dataset | |
| MAPT (microtubule-associated protein tau) PSEN1 (presenilin 1) APP (amyloid beta (A4) precursor protein) |
3.9E-12 1.8E-10 1.2E-09 |
ALDOA↓, BASP1↑, CAP1↓, DPYSL2↓, ENO1↓, HK1↑, HSP90AA1↑, HSPA8↓, PGAM1↓, STXBP1↑ | |
| FMR1 (fragile X mental retardation syndrome 1) | 6.2E-09 | ALDOA↓, BASP1↓, CAMK2A↑, EEF2↑, ENO1↓ | |
| HTT (huntingtin) | 1.3E-03 | BASP1↑, CAMK2A↑, HSPA8↓, NSF↑, STXBP1↑ | |
Focus proteins are in bold font. Other proteins (regular font) are included in networks based on known protein interactions in the Ingenuity Pathway Analysis database. Arrows indicate direction of change in protein expression (↓ downregulated, ↑ upregulated) induced by alcohol drinking, as shown in Table 1. Protein clusters with multiple functions are noted by brackets. p values were derived in Ingenuity Pathway Analysis by right-tailed Fisher exact test and indicate relative overrepresentation of proteins in a given function than what is expected by chance.
LTP, long-term potentiation.