FIG. 4.

Results of apical sampling following brief (7.5 min) injections into the basal turn of ST in three experimental conditions. Individual experiments are shown gray and mean ± standard deviation curves are shown black. Left, injection in “normal” orientation with right, operated ear uppermost. Middle, injection with animal rotated 180 °, with right, operated ear lowest. Right, injection with animal in normal orientation but with the round window covered with Tissumend cyanoacrylate adhesive. Both experimental conditions resulted in sample concentrations that were significantly different from the control condition (two-way ANOVA, Bonferroni, df 109, groups I vs H, t statistic 16.35, P < 0.001; groups J vs H, t statistic 6.37, P < 0.001), with 180 ° rotation resulting in significantly lower concentrations and round window occlusion resulting in significantly higher concentrations.