Figure 2.
The RBM7 RRM domain exhibits specificity for U-containing oligopyrimidin sequences. (A) Schematic representation of the domain organization of NEXT complex subunits. Length of each protein in amino acids is indicated on the right. The boxes mark individual domains or protein regions; RRM, RNA recognition motif, ZnK, zinck knuckle, Pro-rich, proline-rich, N-term, N-terminal, RecA, helicase domain, WH, winged-helix domain, Hel-bundle, helical bundle. (B) Equilibrium binding of the RBM7 RRM with different fluorescently labeled RNA substrates monitored by fluorescence anisotropy. Binding isotherms and dissociation constants (Kd) are shown for each substrate. (C) RBM7 RRM requires minimum 4 nt RNA for binding. Equilibrium binding of the RBM7 RRM with fluorescently labeled polypyrimidin RNA substrates of varying lengths monitored by fluorescence anisotropy. Binding isotherms and dissociation constants (Kd) are shown for each substrate.