Table 1. Summary of the properties of Hop2-Mnd1 and its mutants.
| Hop2-Mnd1 and its mutants | dsDNA binding | ssDNA binding | RAD51 interaction | DMC1 interaction | Reference |
|---|---|---|---|---|---|
| Hop2-Mnd1 | + | + | + | + | 12 |
| Hop2 (K63A/Y65A/K67A)-Mnd1 | - | - | + | + | 12 |
| Hop2-Mnd1 (R64A/Y70A/Y71A) | - | + | + | + | 12 |
| Hop2 (R176A)-Mnd1 | + | - | + | + | 12 |
| Hop2-Mnd1 (KYK+RYY) | - | - | + | + | 12 |
| Hop21-143-Mnd1 | + | - | - | nd | 12 |
| Hop2126-217-Mnd1 | - | - | + | nd | 12 |
| Hop2-Mnd11-113 | + | + | - | nd | 12 |
| Hop2-Mnd1100-205 | - | + | + | nd | 12 |
| Hop2-Mnd11-185 | + | + | - | - | This study |
| Hop21-190-Mnd1 | + | + | - | - | This study |
| Hop2-Mnd1 (F195A) | + | + | - | - | This study |
| Hop2-Mnd1 (I197Q) | + | + | - | - | This study |
| Hop2-Mnd1 (F201A) | + | + | - | - | This study |
| Hop2-Mnd1 (F195A/I197Q/F201A) | + | + | - | - | This study |
| Hop2 (K193A/K195A/K196A)-Mnd1 | nd | nd | +* | nd | This study |
| Hop2 (F198A/F199A)-Mnd1 | + | + | - | - | This study |
| Hop2 (G203N)-Mnd1 | + | + | + | + | This study |
| Hop2 (V202Q/I204Q)-Mnd1 | + | + | - | - | This study |
| Hop2 (V212Q)-Mnd1 | nd | nd | +* | nd | This study |
| Hop2 (L213Q/L214Q)-Mnd1 | nd | nd | +* | nd | This study |
| Hop2 (ΔE201)-Mnd1 | + | + | - | - | This study |
Note: ‘+’ denotes wild-type activity; ‘-’ denotes an impairment of activity; nd = not determined. The asterisk (*) denotes yeast three-hybrid interaction.