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. 2015 Apr 6;43(8):4075–4086. doi: 10.1093/nar/gkv273

Figure 4.

Figure 4.

miR-890 and miR-744–3p directly target MAD2L2 and RAD23B. (A) Western blot assays of four prostate cancer cell lines, C4–2, LNCaP, PC3 and DU145, for MAD2L2 (left) and RAD23B (right) 48 h after 20 nM of miRNA mimetic transfection. Asterisk represents a nonspecific band. (B) Schematic of MAD2L2 and RAD23B 3′UTR miRNA binding sites. Mutated (Mut) MAD2L2 and RAD23B seed sequences are indicated (red). (C) Luciferase activities following transfection with indicated 3′UTR reporters and miRNA mimetics in LNCaP cells. Relative luciferase activity (mean ± SE, n = 4) is normalized to control miRNA for each reporter. *, P < 0.05 relative to control miRNAs.