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. 2015 Apr 6;43(8):4075–4086. doi: 10.1093/nar/gkv273

Figure 5.

Figure 5.

Multiple DDR and DNA repair pathway genes targeted by radiation sensitizing miRNAs. (A) Western blot analyses of MAD2L2, WEE1, XPC, KU80, RAD23B, XLF and MCL1 48 h after LNCaP transfection with 20 nM of miR-890 (left) and miR-744–3p (right). Asterisk represents a nonspecific band. ACTB was utilized as a loading reference. (B) Normalized percent protein knock-down (mean ± SE, n = 3) by miR-890 (left) and miR-744–3p (right), relative to control miRNA (from three separate experiments). (C) Luciferase activities following transfection with indicated 3′UTR reporters and miR-890 or control mimetics in 293T cells. (D) Luciferase activities following transfection with indicated 3′UTR reporters and miR-744–3p or control mimetics in 293T cells. Relative luciferase activity (mean ± SE, n =12) is normalized to control miRNA for each reporter. *, P < 0.05 relative to control miRNAs.