Figure 2.
Transcription-dependent incorporation of wild-type histone H3 in a mutant chromatin background. (A) Similar experiment as in Figure 1 but measuring the incorporation of wild-type H3HA in a chromatin containing histone H3 modification mutants. Yeast strains deleted for both chromosomal H3 and H4 loci and expressing plasmid-encoded histone H4 and either wild-type or mutant histone H3 variants from their native promoters were grown in raffinose and arrested in G1 by alpha factor. Expression of wild-type H3HA was then induced at T0 (min) by adding galactose to the medium. (B) The time course of H3HA incorporation at the indicated loci and histone mutant strains was determined by quantitative ChIP analysis as in Figure 1. ChIP values are expressed as the percentage of input DNA recovered as in Figure 1. A western blot to assess for galactose activation of the H3HA proteins is shown in Supplementary Figure S1.