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. 2015 Apr 6;43(8):3986–3997. doi: 10.1093/nar/gkv283

Figure 1.

Figure 1.

Gadd45a synergizes with Tet and TDG in activation of a methylated reporter gene in a dual luciferase reporter assay. A methylated (A) or unmethylated (B) pCpGL-CMV-firefly luciferase reporter was co-transfected into HEK293T cells with Gadd45a, Tet2CD and TDG in various combinations indicated. Shown is the firefly luciferase activity normalized to the level of control pCpGL-CMV-renilla luciferase relative to that of cells without Tet, TDG and Gadd45 expression, which is set to 1. ‘m’ denotes catalytically inactive mutants of Tet2CD and TDG. Data are represented as the mean ± SEM of three independent experiments.