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. 2015 Apr 6;43(8):3986–3997. doi: 10.1093/nar/gkv283

Figure 2.

Figure 2.

Ectopic expression of Gadd45a reduces genomic 5fC and 5caC generated by Tet2 in transfected HEK293T cells. (A) HPLC analysis of 5caC in the genomic DNA of cells overexpressing full-length Tet2 with and without Gadd45a. The y-axis indicates OD280nm of nucleoside preparations from genomic DNA. Defined nucleosides were used as standards (C, 5mC, 5hmC and 5caC). Dashed lines indicate the elution position of 5caC. (B) Mass spectrometric quantification of modified cytosines (5mC, 5hmC, 5fC and 5caC) in the genomic DNA of cells ectopically expressing Tet2 alone or with Gadd45a. Data are represented as the mean ± SEM from two independent experiments, and are analyzed by two-tailed t-test, P < 0.05 (*).