Skip to main content
. 2015 Mar 30;36(5):564–573. doi: 10.1093/carcin/bgv041

Figure 2.

Figure 2.

E2 specifically decreases the activity of ERK1/2 by non-genomic mechanisms in metastatic granulosa cells. (A) Analyses of the activity of MAPK and PI3K signaling pathways by western blot assays on whole cell lysates of KGN cells after 10 and 60min of E2 treatment. (a) Expression levels of phospho- and total ERK1/2. (b) Expression levels of phospho- and total p38 MAPK. (c) Expression levels of phospho- and total Akt. The ratios of phosphorylated to total levels, which reflect protein kinase activity, were determined after quantification of band intensities. Graphs show the means ± SEM of at least three independent experiments. (B) Time-course study of phospho- and total ERK1/2 levels from 5min to 24h after a 10-nM E2 treatment. (C) Representative western blot assay of phospho- and total MEK levels in KGN cells with or without E2 treatment. Cells were treated by E2 for 15min.