Fig. 3.

Effect of inwardly directed gradients of glutarate, DMPSH, or DMPSS (each in a concentration of 500 µM) on ¹⁴C-glutarate efflux in stably transfected HEK293 cells (rbOAT3, hOAT3). HEK293-OAT cells were preloaded with ¹⁴C-glutarate for 1h at 37°C. The efflux was calculated as percentage of ¹⁴C content recovered in the supernatant compared with the sum of total radioactivity recovered in the medium and that remaining in the cells at the conclusion of a 10 min efflux period at 37°C, where OAT-expressing cells in Ringer’s solution were set to 100%. Data are expressed as means SE of triplicate measurements from 3 separate experiments. If not otherwise noticed all tested substances showed a highly significant (P < 0.001) efflux of [¹⁴C]glutarate. *P < 0.05; **P < 0.01;