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. Author manuscript; available in PMC: 2016 Apr 7.
Published in final edited form as: Cell Rep. 2015 Apr 2;11(1):125–136. doi: 10.1016/j.celrep.2015.03.010

Figure 6. MST1 phosphorylation at serine 438 reduces its dimerization and activity.

Figure 6

A. COS7 cells were transfected with the specified plasmids. MYC was immunoprecipitated and immunoblots for MYC and FLAG are shown. Immunoprecipitate with control IgG was used as a control. B–C. Cardiomyocytes were transduced with ad-LacZ, ad-FLAG-WT-MST1, ad-FLAG-S438A-MST1 or ad-FLAG-S438D-MST1. After 48 hours, the levels of phospho-LATS2 (T1041 homologous), total LATS2 and MST1 were evaluated and representative immunoblots are shown (B) together with quantification analysis of the P-LATS2/LATS2 ratio (C). N=3. D. After 72 hours, cardiomyocyte survival was assessed by CellTiter-Blue assay. N=4. All the data are expressed as mean ± SEM * p<0.05; n.s.= not significant. E. Schema representing the negative regulation of MST1 by mTORC2, which preserves cell survival.