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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Cancer Prev Res (Phila). 2015 Feb 24;8(5):419–430. doi: 10.1158/1940-6207.CAPR-14-0418

Figure 2. 4-HPR disrupts actin cytoskeleton organization.

Figure 2

Actin filaments (F-actin) were labeled with a rhodamine fluorescent probe and visualized under 400X image scale via fluorescent microscopy. 4-HPR-treated samples showed (1) loss of cellular polarity, (2) dissipation of cortical actin networks, and (3) loosening of intercellular junctions. HOK3437, HOK3437 E6/E7 and SCC2095sc all exhibited 4-HPR’s dose-escalating effects manifesting as cytoskeletal rearrangement and/or destabilization of actin filaments. SCC15 appeared to be largely unaffected by 4-HPR. A DAPI counterstain identifies the nuclei.