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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Cancer Prev Res (Phila). 2015 Feb 23;8(5):365–374. doi: 10.1158/1940-6207.CAPR-14-0386

Figure 6.

Figure 6

Cell viability inhibition by ITCs was significantly attenuated by CXCR4 overexpression in PC-3 cells. A, western blot showing effect of PEITC treatment (24 hours) on CXCR4 protein level in Neo_PC-3 and CXCR4_PC-3 cells. B, Effects of PEITC, BITC, and SFN treatments (24 hours) on viability of Neo_PC-3 and CXCR4_PC-3 cells. The results shown are mean ± SD (n = 3). Significantly different compared with acorresponding control, and bbetween Neo_PC-3 and CXCR4_PC-3 cells by one-way ANOVA followed by Bonferroni’s test. C, western blotting for S473 phosphorylated AKT using lysates from Neo_PC-3 or CXCR4_PC-4 after 24 hour treatment with DMSO or specified ITC compound. D, western blotting for phospho-ERK and total ERK using lysates from Neo_PC-3 or CXCR4_PC-4 after 4 hour treatment with DMSO or specified ITC compound. The blots were stripped and re-probed with GAPDH to ensure equal protein loading. Each experiment was repeated at least twice.