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. Author manuscript; available in PMC: 2016 May 15.
Published in final edited form as: J Immunol. 2015 Apr 3;194(10):4767–4776. doi: 10.4049/jimmunol.1402551

FIGURE 6. Endogenous Rictor−/− DC show decreased B7-H1 expression and increased IL-12p70 and IL-23 production.

FIGURE 6

CD11c-specific Rictor−/− mice were adoptively transferred (i.v.) with 1H3.1 TCR-tg CD4+ T cells and 2d later injected i.p. with I-Eα(52–68) peptide, with or without LPS. After 6d, splenic DC were harvested and stained. (A) Expression of I-Ab, CD86 and B7-H1 by bulk CD11c+ cells. (B) Discrimination of splenic DC subsets on the basis of differential B220, CD11c, CD11b, CD8α and PDCA1 expression. Representative plots show percentages in mice injected with peptide alone, together with surface markers (CD86, B7-H1). (C) Expression of B7-H1 by splenic DC subsets following peptide or peptide + LPS injection. (D) Intracellular IL-12p70 and IL-23 expression (representative plots showing percentages of positive cells following peptide + LPS administration). Means +1SD are from 4–5 animals per condition and n = 3 experiments were performed. * P < 0.05.