CD11c-specific Rictor−/− mice were adoptively transferred (i.v.) with 1H3.1 TCR-tg CD4+ T cells, and 2d later received I-Eα(52–68) peptide, with or without LPS (i.p.). After 6d, splenic T cells were harvested and stained for Vβ6-TCR and CD90.1, as well as intracellular cytokines. (A) Representative plots show the percentages of 1H3.1 TCR-tg CD4+ T cells, and (B) their total numbers. (C) Representative plots showing the percentages of positive cells for each cytokine and Foxp3 (FSC on the Y-axis), and (D) their total numbers. Data are from 3–5 animals per condition and n = 2 independent experiments. * P < 0.05.