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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Int J Cardiol. 2015 Jan 28;184:190–197. doi: 10.1016/j.ijcard.2015.01.075

Figure 5. Interaction between hsa-miR-199a-5p and FKBP5 3’fUTR.

Figure 5

A. Schematic of bioinformatically predicted interaction site between hsa-miR-199a-5p and Homo sapiens FKBP5 3’UTR based on microRNA.org.[23] Top panel: wild-type (WT) 3’UTR of FKBP5. Bottom panel: mutant (Mut) 3’UTR of FKBP5 where the seed site for hsa-miR-199a-5p is partially ablated. B. Conservation of the hsa-miR-199a-5p seed site based on TargetScanHuman 6.2.[26] C. 3’UTR luciferase assay showing a relative decrease in the normalized luciferase signal from the WT FKBP5 3’UTR luciferase construct in the presence of hsa-miR-199a-5p but not from the mutant (Mut) construct. N=4 replicates per group. **P<0.01 vs. Scramble.