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. Author manuscript; available in PMC: 2016 May 15.
Published in final edited form as: J Immunol. 2015 Apr 15;194(10):4951–4962. doi: 10.4049/jimmunol.1402431

Figure 2. DUSP3 is strongly expressed in human monocytes and macrophages.

Figure 2

(A) mRNA relative expression level of DUSP3 gene in sorted human PBMC CD4+T, CD8+T, CD19+B, neutrophils (CD15+) and monocytes (CD14+). (B) Microarray analysis of the expression of genes encoding A-typical and typical DSPs in human CD14+ sorted monocytes. CD14 was used as positive control for monocytes expressed mRNA. Data are presented as ratio of the fluorescence intensity for the DSP probe of interest and the mean fluorescence intensity for the housekeeping genes of each sample. A negative value corresponds to an expression bellow background level. Results are presented as mean ± SEM. (C) Western blot analysis of DUSP3 protein expression in sorted human B and T lymphocytes, neutrophils, monocytes and in monocytes-derived-macrophages isolated from peripheral blood of healthy donors. GAPDH expression is shown as a loading control. Densitometric ratios of DUSP3 to GAPDH are shown for each representative western blot.