Skip to main content
. Author manuscript; available in PMC: 2016 May 15.
Published in final edited form as: J Immunol. 2015 Apr 15;194(10):4951–4962. doi: 10.4049/jimmunol.1402431

Figure 3. DUSP3−/− mice are resistant to LPS induced-endotoxemia and to CLP-induced septic shock.

Figure 3

(A) DUSP3+/+ (n=17) and DUSP3−/− (n=19) mice were injected i.p. with 6 mg/kg of LPS. Percent survival was documented daily. (B) Body temperature of DUSP3+/+ and DUSP3−/− mice before, 6 h and 24 h after LPS injection. (C) DUSP3+/+ (n=20) and DUSP3−/− (n=20) mice were subjected to CLP (one puncture with 21-gauge needle). Survival was documented daily for 7 days. Mortality incidence rates were compared using Kaplan-Meir with log rank test performed by GraphPrism. (D) Body temperature of DUSP3+/+ and DUSP3−/− mice before, 6h and 24h after CLP. Results presented are a combination of two independent experiments. (E–J) Cytokine beads array (CBA) for IL-6 and TNF on serum samples from DUSP3−/− and DUSP3+/+ mice at basal levels and 2h, 10h and 24h after i.p. injection of 6mg/kg of LPS (E and H); on serum and peritoneal cavity samples from DUSP3−/− and DUSP3+/+ mice at 6h and 24h after CLP (F and I) and on the peritoneal cavity cell-free liquid (G and J). Results are presented as mean ± SEM. n=5 mice per group/time point. *P<0.05, **P<0.01, ***P<0.001.