Figure 6. DUSP3 deficiency is associated with a dominance of M2-like over M1-like peritoneal macrophages.

Peritoneal cells harvested from PBS treated, LPS (6mg/kg) or CLP challenged DUSP3^−/− (dark bares) and DUSP3^+/+ (open bares) mice and analyzed by flow cytometry for CD11b, F4/80 and Ly6G to discriminate between macrophages (Ly6G/CD11b⁺/F4–80⁺) and neutrophils (Ly6G⁺/CD11b⁺/F4/80⁻). Percentage of macrophages (A) and neutrophils (B) out of total live cells are presented as histogram of means ± SEM. (C–F) Peritoneal cells from PBS treated, LPS (6mg/kg) or CLP challenged DUSP3^−/− and DUSP3^+/+ mice analyzed by flow cytometry to discriminate macrophages subtypes. F4/80^highCD11b^high (M2-like) and F4/80^intCD11b^int (M1-like) were gated out of total live cells extracted from PBS (C), 48h LPS (6mg/kg) challenged (D) or 48h after CLP challenged (E) DUSP3^−/− and DUSP3^+/+ mice. (F) Quantification of M1-like and M2-like cells extracted from PBS, LPS or CLP challenged mice. n: 6 to 10 mice in each group. PMs were extracted from DUSP3^+/+ and DUSP3^−/− mice PBS or LPS treated for 48h (6mg/kg). qRT-PCR was performed to quantify the expression of Arg1 (G), Chi313 (H) and Nos2 (I) and The expression of gene of interest was relative to beta macroglobulin (β2M). n=3 mice in each experimental group. Results are presented as a mean ± SEM. *<0.05; **p<0.01; ***p<0.001.