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. Author manuscript; available in PMC: 2016 May 15.
Published in final edited form as: J Immunol. 2015 Apr 15;194(10):4951–4962. doi: 10.4049/jimmunol.1402431

Figure 7. Adoptive transfer of DUSP3−/− bone marrow or monocytes confers resistance of WT mice to LPS.

Figure 7

(A–B) Percent survival to LPS challenge (A) and body temperature (B) of irradiated WT-CD45.1 mice transplanted with DUSP3−/−-CD45.2 (DUSP3−/−BM>WT) or DUSP3+/+-CD45.2 (WT BM>WT) derived bone marrow (BM) cells. (C–D) Percent survival to LPS challenge (6mg/mL) (C) and body temperature (D) of WT-CD45.1 mice transplanted with monocytes (1.5×106) from DUSP3−/−-CD45.2 (DUSP3−/−>WT) or DUSP3+/+-CD45.2 (WT>WT) mice. Incident of lethality were compared and analyzed using Kaplan-Meir with log rank test performed by graph prism. N=6 mice in each experimental group. (E) Representative FACS dot plots showing the staining for CD11b and F4/80 gated on live singlets peritoneal cavity cells 48 hours after LPS (6mg/kg) challenge of WT>WT and DUSP3−/−>WT. (F) CD45.2 surface expression on gated M1-like and M2-like cells shown in (E). Results are shown as percent of maximum (% of Max) of overlayed histograms of the gated populations shown in (E).