Fig. 5.

Increased La_13–27 and snRNP_357–373 tetramer⁺ MZ-P and T3 B cells in BXD2 mice. A Spleen cells from 6–8 month old B6 and BXD2 mice were tetramer stained and enriched for FACS analysis of the frequency and number of La_13–27 or snRNP_357–373 tetramer⁺ B cells. B Cell counts for La_13–27 and snRNP_357–373 tetramer⁺ cells in total single cell suspension derived from the spleens of B6 and BXD2 mice. C La_13–27 and snRNP_357–373 tetramer⁺ cells were further analyzed for the frequency of IgM^hiCD21^hi B cells or IgM^lo/−CD21^lo/− B cells (top panels). The IgM^hiCD21^hi B cells were further gated into IgM^hiCD21^hi CD23⁻ MZ and IgM^hiCD21^hi CD23⁺ MZ-Ps (bottom panels), and the frequency of MZ or MZ-P B cells within this population is shown. (D Cell counts for La_13–27 and snRNP_357–373 tetramer⁺ MZ and MZ-P cells in spleens of B6 and BXD2 mice (per 10⁵ events analyzed). E FACS analysis showing the frequency of La_13–27 and snRNP_357–373 tetramer⁺ transitional B cell subsets. F Cell counts for La_13–27 and snRNP_357–373 tetramer⁺ T1, T2, and T3 B cells in spleens of B6 and BXD2 mice. Each panel is representative of 3–5 mice and at least two independent experiments. * P < 0.05 and ** P < 0.01 between B6 and BXD2.