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. Author manuscript; available in PMC: 2016 May 15.
Published in final edited form as: J Immunol. 2015 Apr 13;194(10):4676–4687. doi: 10.4049/jimmunol.1402870

Figure 8.

Figure 8

rHDL inhibits activation of BMDCs by interfering with the TLR4 pathway. WTBMDCs were stimulated with LPS (0.5 μg/mL) and treated with 4 μM rHDL for the indicated time periods. (A) Confocal immunofluorescence microscopy for p65 (red) translocation into the nucleus (DAPI stained, blue) in BMDCs stimulated with LPS for 2 h. Maximum projections of image stacks are shown; representative of two independent experiments. (B) Quantification of p65 cell distribution by determining nuclear: cytoplasmic (N: C) ratios of signal intensity at single cell level. Data are combined of two independent experiments; ***p<0.0001. (C-E) Relative mRNA levels of Myd88 (***p=0.0004), Trif and Atf3 (*p=0.0177, **p=0.0016) after treatment of WT BMDCs (n=6-8) with rHDL for 4h and 18h. Results are expressed as mean ± SEM; data are combined of at least three independent experiments.