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. Author manuscript; available in PMC: 2016 May 15.
Published in final edited form as: J Immunol. 2015 Apr 15;194(10):4621–4630. doi: 10.4049/jimmunol.1402493

FIGURE 3.

FIGURE 3

Role of PAR-2 in the induction of lung ILC2 and eosinophilia promoted by airway exposure to subtilisin. (A) To analyze lung group 2 innate lymphoid cells (ILC2), C57BL/6 WT or PAR-2-/- mice received i.n. subtilisin for three consecutive days and lung cells were analyzed on day 4. (B) FACS plots show lung ILC2, as defined by CD45+Lin-Thy-1.2+, after ex vivo stimulation with PMA and ionomycin to induce cytokine production. (C) Experimental protocol used to promote allergic inflammation by airway sensitization to subtilisin. WT, total PAR-2-/-, and the mixed chimera (WT BM to PAR-2-/- and PAR-2-/- BM to WT) mice were i.n. sensitized with 1 μg subtilisin on days 0 and 7, and samples were harvested on day 8. (D) Number of BAL eosinophils from subtilisin-administered mice. Error bars show *, P ≤ 0.01, for significant differences to WT group. Data are mean ± SEM and are representative of two independent experiments (n=5-9 mice per group).