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. Author manuscript; available in PMC: 2016 May 15.
Published in final edited form as: J Immunol. 2015 Apr 17;194(10):4759–4766. doi: 10.4049/jimmunol.1402954

Figure 5. p38α signaling in hepatocytes is dispensable in TNF-induced cell death.

Figure 5

(A & B) Hepatocytes from p38αfl/fl or p38αΔliver mice (A), or control or p38α KD Hepa 1-6 hepatocyte cells (B) were incubated with actinomycin D (ActD, 1 μg/ml) and TNF. Cell viability was assessed after 24 h. (C & D) Control or p38α KD Hepa 1-6 cells were incubated with ActD and TNF (30 ng/ml). Cell lysates were prepared at the indicated times to examined the activation of caspases (C) or NF-κB/MAPK signaling (D). Knockdown of p38α was confirmed using anti-p38α antibody. GAPDH levels were determined as an internal control.