Figure 1. Schematic of oncolytic adenoviruses expressing interferon alpha.
Both viruses have a RGD-4C motif incorporated into the fiber knob region for infectivity enhancement. Additionally, an expression cassette containing the adenovirus death protein (ADP) and the hamster interferon alpha transgene (IFN) were placed into the adenovirus E3 region. In the CRAd, the Cox2 promoter was inserted into the 5’-side of the adenovirus E1 region to serve as a tissue-specific promoter.