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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Surgery. 2015 Feb 27;157(5):888–898. doi: 10.1016/j.surg.2015.01.006

Figure 2. Syngeneic immunocompetent hamster model.

Figure 2

A. The Cox2 promoter activity of five hamster pancreatic cancer cell lines (Hap-T1, HP-1, Taka-1, PGHam-1, and PC-1) was analyzed. The A549 (human lung adenocarcinoma) cell line was used as a Cox2-positive control, and results were reported in relative light units (RLU) with respect to the A549 cells. All cell lines demonstrated Cox2 promoter activity, with the highest being in the Hap-T1 and HP-1 cells.

B. Ability of human adenovirus to replicate in hamster pancreatic cancer cell lines. The RGD-Cox2-ΔE3-ADP-Luc virus was added at various concentrations and the crystal violet assay was used to qualitatively analyze cell viability. In the HP-1 and Hap-T1 cell lines, even low titers of 10 vp/cell were able to induce total cell death. At high viral titers, the virus achieved a complete cytocidal effect in all cell lines.

C. Active replication of a Cox2 promoter controlled adenovirus (RGD-Cox2-ΔE3-ADP-Luc) in a hamster model was demonstrated via bioluminescent imaging. Following a single intratumoral injection, a signal was evident as early as the day after inoculation and persisted for up to 4 weeks (day 5 image shown).