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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Surgery. 2015 Feb 27;157(5):888–898. doi: 10.1016/j.surg.2015.01.006

Figure 3. Cytocidal effect and species specificity of IFN-expressing vectors.

Figure 3

One human pancreatic cancer cell line (MIA PaCa-2) and two hamster pancreatic cancer cell lines (HP-1 and PGHam-1) were infected with three non-replicating adenoviruses that only differed in the transgene that they expressed (RGD-nonrep-Luc, RGD-nonrep-hu-IFN, RGD-nonrep-ham-IFN). Gradually increasing doses of virus were used (102, 103, and 104 vp/cell), and the percentages of living cells were quantified using the MTS assay four days after viral infection. Results are shown as a percentage of living cells.

In the MIA PaCa-2 cell line, the human IFN-expressing adenovirus demonstrated a significant cytocidal effect when compared to the luciferase control vector (* p<0.05), while the hamster IFN-expressing vector demonstrated minimal cell death. Conversely, in the HP-1 and PGHam-1 cell lines, the hamster IFN-expressing adenovirus resulted in significant cell death (* p<0.05) and greatly outperformed its human IFN-expressing counterpart.