Figure 4. In vivo hematopoietic reconstitution potential of ex vivo expanded MPB cells.

A) In vivo hematopoietic reconstitution capacity of 5azaD/TSA expanded MPB cells in NSG mice. NSG mice were transplanted with CD34+ cells containing 5 × 10⁴ CD34⁺CD90⁺ cells from uncultured CD34+ MPB cells or the product of an equal initial number of CD34+CD90+ cells after 9 days of culture in the presence of 5azaD/TSA and cytokine A or Cytokine A alone. Similar rates of engraftment were observed between equal doses of CD34⁺CD90⁺ from primary and 5azaD/TSA expanded MPB cells. B) Representative flow cytometry analysis of mice transplanted with MPB cells expanded in the presence of 5azaD/TSA or control cultures. Bone marrow cells were stained with fluorochrome conjugated antibodies against human CD45, CD19, CD33, CD34 and CD41 to determine multilineage human hematopoietic engraftment.