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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Transfusion. 2014 Nov 2;55(4):864–874. doi: 10.1111/trf.12904

Table 2.

Colony forming unit potential of 5azaD/TSA expanded mobilized peripheral blood cells

Mean number of CFU / 500 cells plated
Culture
Condition
CFU-GM BFU-E CFU-mix Total CFU Plating
Efficiency (%)
Fold Expansion
of CFU-mix
Day 0
Primary MPB
64.0 ± 11.8 75.2 ± 0.2 2.7 ± 0.8 141.8 ± 10.9 28.4 ± 2.2 n/a
Day 9
Cytokine A
38.2 ± 8.7 3.5 ± 0.8 0 41.7 ± 9.4 8.3 ± 1.9 0
Day 9
5azaD/TSA +
Cytokine A
49.2 ± 6.3 8.2 ± 1.1 3 ± 0 60.3 ± 6.3 10.4 ± 1.9 10.1 ± 0.5
Day 9
Cytokine B
14.0 ± 0.7 1.3 ± 0.2 0 15.3 ± 0.5 3.1 ± 0.1 0
Day 9
5azaD/TSA +
Cytokine B
33.5 ± 9.2 6.5 ± 0.4 2.25 ± 0.2 42.3 ± 9.0 8.5 ± 1.8 13.0 ± 0.2

MPB = mobilized peripheral blood; CFU = colony-forming unit; BFU = burst-forming unit; 5azaD = 5-aza-2’-deoxyctidine (5azaD); TSA = trichostatin A Cytokine A and B represent cytokine conditions with the highest and lowest expansion of CD34+CD90+ cord blood cells, respectively1