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. Author manuscript; available in PMC: 2015 May 3.
Published in final edited form as: Pharmacogenet Genomics. 2009 Jan;19(1):87–89. doi: 10.1097/FPC.0b013e32830fbde4

Figure 4.

Figure 4

Biochemical properties of the in vitro O-methylation of catechol substrates with respect to AdoMet and AdoHcy concentrations. The representative substrates used in these assays were 2-OH-E2 and 4-OH-E2 at 10 µM concentration. The incubation mixture consisted of the substrate, 100 µM [methyl-3H]AdoMet (containing 0.2 µCi or as indicated), 16.2 µg/mL of wild-type S-COMT (A) or mutant S-COMT (B), 1 mM 1,4-dithiothreitol, and 1.2 mM MgCl2 in a final volume of 300 µL Tris-HCl buffer (50 mM) at pH 7.4. The incubations were carried out at 37°C for 15 minutes. Each value is the mean of duplicate measurements.