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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Biomaterials. 2015 Apr 17;56:129–139. doi: 10.1016/j.biomaterials.2015.03.041

Figure 4. The effects of added ECM in 3D HA hydrogel on drug efficacy: tumor growth (cluster size) and apoptosis (# of clusters).

Figure 4

Cells were grown for 3 days in 3D HA with no RGD, 1.2 μM, 12 μM RGD, Fibronectin, or Laminin and then were treated for 3 days with 30 μM Dabrafenib (BRAF-i), VX11e (ERK-i) or.1 % DMSO control. Cells were than stained live (green, cytoplasm)/dead (red, DNA), and cluster size and cluster number were measured, to determine the effect of the addition of soluble RGD or ECM proteins. * indicates p<0.05, ** indicates p <0.01, *** indicates p <0.001, **** indicates a p<0.0001, compared using ANOVA. Only A375P and WM793 had significant effects from RGD treatment. 2 independent experiments for each cell line, 4 independent fields of view per experiment, N values of >500 cells.