Misoprostol treatment decreases HMGB1 expression, Src kinase activity, and IL-1β expression in the collagenase-induced ICH model. (A–D) Western blot analysis shows the effects of misoprostol treatment on levels of HMGB1 (A), phosphorylated (P−) and total Src (B), COX-2 (C), and IL-1β (D) in the hemorrhagic hemisphere on day 1 after ICH. β-actin and total Src were used as loading controls. Misoprostol treatment reduced immunoreactivity of HMGB1, phosphorylated Src, and IL-1β, but not COX-2 on day 1 after ICH. O.D., optical density. n=5 mice/group; All values are means ± SD; *p<0.05, **p<0.01, t-test.