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The effect of 1,25(OH)2D3 on the expression of VDR and PPARγ. RAW264.7 cells were stimulated with 1,25(OH)2D3 in a dose- (0.1 nM, 1 nM, 10 nM, and 100 nM) dependent manner. After 24 h, the cells were collected for (a) RT-PCR and (b) western blotting analysis. β-actin was used as an internal control. A concentration of 11.1 mM glucose was used as a control. HG: 25 mM glucose. Data are presented as the mean ± SD (n = 3-4 per group). * P < 0.05 versus control; # P < 0.05 versus HG.
