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The effect of a PPARγ antagonist (GW9662) on iNOS and MR expression. RAW264.7 cells were treated with GW9662 for 2 h before administering 10−8 M 1,25(OH)2D3 to high glucose-pretreated macrophages. The cells were collected for (a) RT-PCR, (b) western blotting, and (c) immunofluorescence analysis (200x). β-actin was used as an internal control. DMSO was used as a negative control. Data are presented as the mean ± SD (n = 3-4 per group). * P < 0.05 versus VD group.
