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The effect of VDR siRNA on iNOS, MR, and PPARγ expression. VDR siRNA pretreatment with cells was performed before administering 10−8 M 1,25(OH)2D3 to high glucose-treated macrophages. The cells were collected for (a) iNOS, MR, and (b) PPARγ analysis. β-actin was used as an internal control. NTC: nontarget control. Data are presented as the mean ± SD (n = 3-4 per group). * P < 0.05 versus VD group.
