Table 2.
The mRNA expression of M1/M2 macrophage-specific markers of each group (RT-PCR).
| Control | HG | M1 model | Mannitol | VD | M2 model | |
|---|---|---|---|---|---|---|
| iNOS | 0.02 ± 0.005 | 0.39 ± 0.11* | 0.43 ± 0.15* | 0.01 ± 0.003 | 0.09 ± 0.04# | 0.04 ± 0.02# |
| MR | 2.39 ± 0.36 | 1.97 ± 0.13* | 1.43 ± 0.30* | 2.26 ± 0.54 | 2.74 ± 0.03# | 3.54 ± 0.12# |
| Arg-1 | 10.24 ± 0.03 | 2.93 ± 0.14* | 2.29 ± 0.24* | 10.04 ± 0.56 | 8.20 ± 0.68# | 8.82 ± 1.34# |
Control: 11.1 mM glucose; HG: 25 mM glucose; M1 model: 100 U/mL IFNγ + 5 ng/mL LPS; VD: 25 mM glucose + 10−8 mol/L 1,25(OH)2D3; M2 model: 10 ng/mL IL-4. Data are presented as the mean ± SD (n = 3-4 per group). * P < 0.05 versus control; # P < 0.05 versus HG.