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. Author manuscript; available in PMC: 2015 Nov 15.
Published in final edited form as: J Immunol. 2015 Apr 3;194(10):4705–4716. doi: 10.4049/jimmunol.1402979

Figure 3.

Figure 3

Cyp2j4 deletion results in a pro-fibrotic macrophage transcriptome enriched for PPARγ targets. (A) Differential expression analysis of RNA-sequencing of WT and Cyp2j4−/− BMDMs (FDR<0.05) revealed an up-regulation of collagen family genes (upper-left panel), TGFβ signalling genes (upper-right panel) and ECM/collagen-associated genes (lower-left panel). *, P < 0.01 compared to WT. (B) KEGG pathway analysis showed markedly significant representation of ECM-receptor interaction and Focal adhesion pathways. (C) Western Blot analysis showing enhanced PPARγ protein levels in Cyp2j4−/− BMDMs. (D) Heat map showing the enrichment for PPARγ targets (59 out of 259 differentially expressed genes, P=2.5 10−17) among the 259 differentially expressed genes (FDR < 0.05) between WT and Cyp2j4−/− BMDMs.